Caspase8 (DED)
- At a glance
- In detail
- Full Name:
- Caspase-8
- Alternative Names:
- CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, FLJ17672, MGC78473, CASP8
- Function:
- Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
- Death Domain Subfamily & Boundary Region:
-
DED
:
2-80, 100-177
Species |
External DB
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Uniprot | DIP | STRING | KEGG | IntAct | MINT | ||
Human |
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Q14790![]() |
DIP-30915N![]() |
Q14790![]() |
hsa:841![]() |
Q14790![]() |
MINT-91645![]() |
Mouse |
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O89110![]() |
DIP-37435N![]() |
O89110![]() |
mmu:12370![]() |
O89110![]() |
MINT-91569![]() |
Rat |
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Q9JHX4![]() |
Q9JHX4![]() |
rno:64044![]() |
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Dog |
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Q38JA9![]() |
Q38JA9![]() |
cfa:488473![]() |
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Zebra fish |
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Q9I8J3![]() |
Q9I8J3![]() |
Not solved yet
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In vitro interaction | |||||||||||||
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DD1 | DD2 | Reference | |||||||||||
Family | DD1 | DD2 | Method | Species | Region | Expression | Species | Region | Expression | ||||
DED | Caspase8 |
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Caspase8 | Yeast two-hybrid | Mouse | 1-202 | Yeast | Mouse | 1-202 | Yeast | 10526240 | ||
DED | Caspase8 |
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Caspase8 | Yeast two-hybrid | Mouse | 1-202 | Yeast | Mouse | 82-202 | Yeast | 10526240 | ||
DED | Caspase8 |
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DEDD | GST fusion protein pull-down | Not specified | Full length | In vitro translation | Human | 1-318 | In vitro translation | 9774341 | ||
DED | Caspase8 |
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DEDD | GST fusion protein pull-down | Not specified | 1-180 | In vitro translation | Human | 1-318 | In vitro translation | 9774341 | ||
DED | Caspase8 |
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DEDD | GST fusion protein pull-down | Not specified | 1-201 | In vitro translation | Not specified | Full length | E.coli | 11965497 | ||
DED | Caspase8 |
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DEDD2 | GST fusion protein pull-down | Not specified | 1-201 | In vitro translation | Not specified | Full length | E.coli | 11965497 | ||
DED | Caspase8 |
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FADD | Fluorescence resonance energy transfer | ` | 11717445 | |||||||
DED | Caspase8 |
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FADD | GST fusion protein pull-down | HuH-7 lysate | Not specified | Full length | E.coli | 12407100 | ||||
DED | Caspase8 |
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FADD | His fusion protein pull-down | Not specified | 1-479 | In vitro translation | Not specified | Full length | Not specified | 8681377 | ||
DED | Caspase8 |
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FADD | His fusion protein pull-down | Human | Not specified | In vitro translation | Not specified | Not specified | Not specified | 9045686 | ||
DED | Caspase8 |
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FADD | His fusion protein pull-down | Human | 1-479 | In vitro translation | Not specified | Not specified | In vitro translation | 12010809 | ||
DED | Caspase8 |
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FADD | In vitro purification protein assembly(2D gel electrophoresis) | Not specified | 1-479 | In vitro translation | Not specified | Full length | HEK293 | 8681377 | ||
DED | Caspase8 |
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FADD | Yeast two-hybrid | Human | Not specified | Yeast | Human | Full length | Yeast | 8681376 | ||
DED | Caspase8 |
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FADD | Yeast two-hybrid | Human | Not specified | Yeast | Human | 1-79 | Yeast | 8681376 | ||
DED | Caspase8 |
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FADD | Yeast two-hybrid | Mouse | 1-202 | Yeast | Mouse | 1-96 | Yeast | 10526240 | ||
DED | Caspase8 |
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FADD | Yeast two-hybrid | Human | Full length | Yeast | Not specified | Not specified | Yeast | 15782135 | ||
DED | Caspase8 |
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FLIP | GST fusion protein pull-down | Not specified | 1-201 | In vitro translation | Human | Full length | Not specified | 9228018 | ||
DED | Caspase8 |
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FLIP | Yeast two-hybrid | Human | Full length | Yeast | Human | 1-215 | Yeast | 9208847 | ||
DED | Caspase8 |
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FLIP | Yeast two-hybrid | Not specified | 1-201 | Yeast | Human | 1-160 | Yeast | 9228018 | ||
DED | Caspase8 |
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FLIP | Yeast two-hybrid | Not specified | Not specified | Yeast | Human | Full length | Yeast | 9289491 | ||
DED | Caspase8 |
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FLIP | Yeast two-hybrid | Not specified | 1-480 | In vitro translation | Human | 1-208 | Not specified | 9326610 | ||
DED | Caspase8 |
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FLIP | Yeast two-hybrid | Not specified | 1-216 | In vitro translation | Human | 1-180 | Not specified | 9326610 |
(
: click this icon to show interactions only between the two corresponding DDs)

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In vivo interaction | |||||||||||||
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Endogenous expression |
Overexpression | DD1 | DD2 | Reference | |||||||||
Family | DD1 | DD2 | Method | Species | Region | Species | Region | ||||||
DED | Caspase8 |
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Caspase10 | Co-immunoprecipitation | HT1080 | 12887920 | |||||||
DED | Caspase8 |
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Caspase10 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | Not specified | 12887920 | |||
DED | Caspase8 |
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Caspase10 | Co-immunoprecipitation | SKW6.4 | 17159907 | |||||||
DED | Caspase8 |
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Caspase10 | Fluorescence resonance energy transfer | Jurkat | Not specified | Full length | Not specified | Full length | 11717445 | |||
DED | Caspase8 |
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Caspase8 | Fluorescence resonance energy transfer | Jurkat | Not specified | Full length | Not specified | Full length | 11717445 | |||
DED | Caspase8 |
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DEDD | Co-immunoprecipitation | HEK293 | Not used | Human | 1-318 | 9774341 | ||||
DED | Caspase8 |
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DEDD | GST fusion protein pull-down | HEK293 | Not specified | Not specified | Not specified | Not specified | 11741985 | |||
DED | Caspase8 |
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DEDD2 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | Full length | 11965497 | |||
DED | Caspase8 |
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DEDD2 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | Full length | 12527898 | |||
DED | Caspase8 |
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DEDD2 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | 1-115 | 12527898 | |||
DED | Caspase8 |
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FADD | HSC3 | 21525013 | ||||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HEK293 | Human | Not specified | Human | Not specified | 8681376 | |||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | T47D | Not specified | Not specified | Human | Not specified | 9582077 | |||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | Jurkat A3 | 11432804 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | MCF-7 | Human | 1-276 | Not used | 12010809 | ||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HEK293 | Human | 2-177 | Human | 2-208 | 12220669 | |||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HT1080 | 12887920 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | Full length | 15701651 | |||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | SKW6.4 | 17159907 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | H2009 | 18485876 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | Panc-1 | 18485876 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HT-1080 | 19060883 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HT-29 | 19524512 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | H2009 | 20547836 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | SH-EP | 22890322 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | LAN-5 | 22890322 | |||||||
DED | Caspase8 |
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FADD | Fluorescence resonance energy transfer | HEK293 | Not specified | Full length | Not specified | Not specified | 11717445 | |||
DED | Caspase8 |
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FLIP | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | Full length | 9208847 | |||
DED | Caspase8 |
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FLIP | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | Full length | 9211860 | |||
DED | Caspase8 |
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FLIP | Co-immunoprecipitation | HEK293 | Not specified | 1-201 | Human | Full length | 9228018 | |||
DED | Caspase8 |
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FLIP | Co-immunoprecipitation | HEK293 | Human | Not specified | Not specified | Not specified | 9380701 | |||
DED | Caspase8 |
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FLIP | Co-immunoprecipitation | Raji cell | Not used | Not specified | Not specified | 12215447 | ||||
DED | Caspase8 |
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FLIP | Co-immunoprecipitation | HT1080 | 12887920 | |||||||
DED | Caspase8 |
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FLIP | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | Not specified | 12887920 | |||
DED | Caspase8 |
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FLIP | Co-immunoprecipitation | SKW6.4 | 17159907 | |||||||
DED | Caspase8 |
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PEA15 | Co-immunoprecipitation | HeLa | Human | Not specified | Human | Full length | 10442631 |
(
: click this icon to show interactions only between the two corresponding DDs)

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Characterization | |||||||||||
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Stoichiometry | Affinity | Protein-Protein interface | Reference | ||||||||
Family | DD1 | DD2 | Method | Mutation | Complex structure | ||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | F25G, F35V (FADD) | 9582077 | |||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | K33Q and K35Q, F25G (FADD) | 12220669 |
(
: click this icon to show interactions only between the two corresponding DDs)

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Functional Role | |||||||||||
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Family | DD1 | DD2 | Method | Death-related | Death-unrelated | Reference | |||||
DED | Caspase8 |
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FADD | β-galactosidase staining | Apoptosis (DISC) | 8681376 | |||||
DED | Caspase8 |
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FADD | DNA fragmentation assay | Apoptosis (DISC) | 11432804 | |||||
DED | Caspase8 |
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FADD | Propidium iodide staining | Apoptosis (DISC) | 12010809 | |||||
DED | Caspase8 |
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FADD | Hoechst staining | Apoptosis (DISC) | 12887920 | |||||
DED | Caspase8 |
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FADD | PARP cleaved assay | Apoptosis (DISC) | 18485876 | |||||
DED | Caspase8 |
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FADD | MTT assay | Apoptosis (DISC) | 22890322 | |||||
DED | Caspase8 |
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FADD | DAPI staining | Apoptosis (DISC) | 9045686 | |||||
DED | Caspase8 |
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FLIP | β-galactosidase staining | Apoptosis inhibition | 9228018 | |||||
DED | Caspase8 |
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FLIP | Confocal microscopy | Apoptosis inhibition | 12215447 | |||||
DED | Caspase8 |
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PEA15 | PARP cleaved assay | Apoptosis inhibition | 10442631 |
(
: click this icon to show interactions only between the two corresponding DDs)
