Myd88 (DD)
- At a glance
- In detail
- Full Name:
- Myeloid differentiation primary response protein MyD88
- Alternative Names:
- MYD88D
- Function:
- Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
- Death Domain Subfamily & Boundary Region:
-
DD
:
54-109
Species |
External DB
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Uniprot | DIP | STRING | KEGG | IntAct | MINT | ||
Human |
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Q99836![]() |
DIP-31349N![]() |
Q99836![]() |
hsa:4615![]() |
Q99836![]() |
MINT-97233![]() |
Mouse |
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P22366![]() |
P22366![]() |
mmu:17874![]() |
P22366![]() |
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Rat |
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Q6Y1S1![]() |
Q6Y1S1![]() |
rno:301059![]() |
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Dog |
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F1P7V2![]() |
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Zebra fish |
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Q5XJ85![]() |
Q5XJ85![]() |
dre:403145![]() |
Structure | Species | Residue | Method | Expression | Purification | Column | Final condition | Final concentration | Reference | |
---|---|---|---|---|---|---|---|---|---|---|
3MOP |
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Human | 20-117 | X-ray | E.coli | Gel filtration | Superdex 200 | 1mg/ml | 20485341 |
Disease | Mutation in disease | Reference |
---|---|---|
Myd88 deficiency | L93P, R196C, E52del | 18669862 |
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In vitro interaction | |||||||||||||
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DD1 | DD2 | Reference | |||||||||||
Family | DD1 | DD2 | Method | Species | Region | Expression | Species | Region | Expression | ||||
DD | Myd88 |
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IRAK2 | GST fusion protein pull-down | Not specified | 1-296 | E.coli | Not specified | Full length | In vitro translation | 12659850 | ||
DD | Myd88 |
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IRAK2 | GST fusion protein pull-down | Not specified | 1-109 | E.coli | Not specified | Full length | In vitro translation | 12659850 | ||
DD | Myd88 |
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IRAK2 | GST fusion protein pull-down | Not specified | 1-155 | E.coli | Not specified | Full length | In vitro translation | 12659850 | ||
DD | Myd88 |
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IRAK4 | His fusion protein pull-down | Human | 1-157 | E.coli | Human | 1-185 | E.coli | 19167362 | ||
DD | Myd88 |
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IRAK4 | In vitro purification protein assembly(chemical crosslinking) | Human | 1-110 | E.coli | Human | 1-152 | E.coli | 19592493 | ||
DD | Myd88 |
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IRAK4 | In vitro purification protein assembly(Size-exclusion chromatography) | Human | 1-110 | E.coli | Human | 1-152 | E.coli | 19592493 | ||
DD | Myd88 |
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IRAK4 | In vitro purification protein assembly(Size-exclusion chromatography) | Human | 1-157 | E.coli | Human | 1-117 | E.coli | 20966070 | ||
DD | Myd88 |
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IRAK4 | Yeast two-hybrid | Not specified | Full length | Yeast | Not specified | Full length | Yeast | 12538665 | ||
DD | Myd88 |
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IRAK4 | Yeast two-hybrid | Not specified | 1-172 | Yeast | Not specified | Full length | Yeast | 12538665 | ||
DD | Myd88 |
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Myd88 | GST fusion protein pull-down | Mouse | Full length | Not specified | Mouse | Full length | In vitro translation | 9575168 | ||
DD | Myd88 |
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Myd88 | Yeast two-hybrid | Mouse | Full length | Yeast | Mouse | Full length | Yeast | 9575168 | ||
DD | Myd88 |
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Myd88 | Yeast two-hybrid | Not specified | Full length | Yeast | Not specified | Full length | Yeast | 11544529 |
(
: click this icon to show interactions only between the two corresponding DDs)

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In vivo interaction | |||||||||||||
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Endogenous expression |
Overexpression | DD1 | DD2 | Reference | |||||||||
Family | DD1 | DD2 | Method | Species | Region | Species | Region | ||||||
DD | Myd88 |
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FADD | Co-immunoprecipitation | HEK293 | Human | Full length | Not used | 10880445 | ||||
DD | Myd88 |
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FADD | Co-immunoprecipitation | HEK293 | Human | 1-151 | Not used | 10880445 | ||||
DD | Myd88 |
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FADD | Co-immunoprecipitation | Drosophila S2 | Drosophila | 1-237 | Drosophila | Not specified | 11606776 | |||
DD | Myd88 |
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FADD | Co-immunoprecipitation | BALB/C Mouse peritoneal macrophage | 21131052 | |||||||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Not specified | 1-151 | Not specified | Full length | 9430229 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Not specified | 1-151 | Not specified | Full length | 10383454 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | THP-1 | 10811644 | |||||||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Not specified | 1-172 | Not specified | Not specified | 10854325 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Human | Full length | Human | 1-215 | 11960013 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | 11976320 | |||||||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | PC12 | 12034707 | |||||||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Human | Not specified | Human | Not specified | 16024789 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | Full length | 17276401 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | 2-109 | 17276401 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | Rat thoracic aortas derived vascular smooth muscle cell | Not specified | 1-306 | Not specified | 1-710 | 17510469 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | MT2 | 17920759 | |||||||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HUT102 | 17920759 | |||||||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | MT4 | 17920759 | |||||||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | 2-712 | 18524972 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | Not specified | 19679662 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | 27-72 | 19679662 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | HEK293 | Not specified | 27-72 | Not specified | Not specified | 19679662 | |||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | GCB DLBCL line BAJB | Not specified | Not specified | Not used | 21179087 | ||||
DD | Myd88 |
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IRAK2 | Co-immunoprecipitation | HEK293 | Human | Full length | Human | Full length | 9374458 | |||
DD | Myd88 |
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IRAK2 | Co-immunoprecipitation | HEK293 | Not specified | 1-151 | Not specified | Full length | 10383454 | |||
DD | Myd88 |
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IRAK2 | His fusion protein pull-down | E.coli | Human | 20-117 | Human | 1-112 | 20485341 | |||
DD | Myd88 |
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IRAK3 | Co-immunoprecipitation | HEK293 | Not specified | 1-151 | Human | Full length | 10383454 | |||
DD | Myd88 |
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IRAK4 | Co-immunoprecipitation | HEK293 | Human | Full length | Human | 1-215 | 11960013 | |||
DD | Myd88 |
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IRAK4 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | Not specified | 15905496 | |||
DD | Myd88 |
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IRAK4 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | Not specified | 19679662 | |||
DD | Myd88 |
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IRAK4 | Co-immunoprecipitation | HEK293 | Human | 1-157 | Human | 1-117 | 20966070 | |||
DD | Myd88 |
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IRAK4 | Co-immunoprecipitation | GCB DLBCL line BAJB | Not specified | Not specified | Not used | 21179087 | ||||
DD | Myd88 |
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IRAK4 | His fusion protein pull-down | E.coli | Human | 20-117 | Human | 4-106 | 20485341 | |||
DD | Myd88 |
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Myd88 | Co-immunoprecipitation | HEK293 | Mouse | 1-296 | Mouse | 1-296 | 11909531 | |||
DD | Myd88 |
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Myd88 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | Not specified | 19679662 | |||
DD | Myd88 |
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Myd88 | Co-immunoprecipitation | HEK293 | Human | 1-157 | Human | 1-157 | 20966070 | |||
DD | Myd88 |
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Myd88 | Size-exclusion chromatography | HEK293 | Mouse | Full length | Mouse | Full length | 9575168 | |||
DD | Myd88 |
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NGFR | Co-immunoprecipitation | PC12 | 12034707 |
(
: click this icon to show interactions only between the two corresponding DDs)

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Characterization | |||||||||||
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Stoichiometry | Affinity | Protein-Protein interface | Reference | ||||||||
Family | DD1 | DD2 | Method | Mutation | Complex structure | ||||||
DD | Myd88 |
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IRAK1 | Co-immunoprecipitation | E52A and E53A, Y58A, K95A (Myd88) | 19679662 | |||||
DD | Myd88 |
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IRAK2 | Structure | 6:4:4 | 3MOP | 20485341 | ||||
DD | Myd88 |
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IRAK4 | Co-immunoprecipitation | E52A and E53A, Y58A, K95A (Myd88) | 19679662 | |||||
DD | Myd88 |
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IRAK4 | Co-immunoprecipitation | S34Y, R98C (Myd88) | 20966070 | |||||
DD | Myd88 |
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IRAK4 | Structure | 6:4:4 | N16E and R20E, E69K, T76E, N78E, R12A (IRAK4) V43D, E52K, I61A, Y58A and R62A, R62E, A44E (Myd88) | 3MOP | 20485341 | |||
DD | Myd88 |
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IRAK4 | Gel filtration | 4:7, 4:8 | 19592493 | |||||
DD | Myd88 |
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IRAK4 | Gel filtration | 7:4, 8:4 | 19592493 | |||||
DD | Myd88 |
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IRAK4 | In vitro purification protein assembly(Size-exclusion chromatography) | S34Y, R98C (Myd88) | 20966070 |
(
: click this icon to show interactions only between the two corresponding DDs)

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Functional Role | |||||||||||
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Family | DD1 | DD2 | Method | Death-related | Death-unrelated | Reference | |||||
DD | Myd88 |
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IRAK1 | Luciferase assay | NF-kB activation | 9430229 | |||||
DD | Myd88 |
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IRAK1 | Luciferase assay | NF-kB-activation | 19679662 | |||||
DD | Myd88 |
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IRAK2 | Luciferase assay | NF-kB activation (Myddosome) | 9374458 | |||||
DD | Myd88 |
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IRAK4 | Luciferase assay | NF-kB activation (Myddosome) | 20966070 | |||||
DD | Myd88 |
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IRAK4 | Kinase assay | IRAK4 mediated IRAK1 phosphorylation | 12538665 |
(
: click this icon to show interactions only between the two corresponding DDs)
