RIG-1 (CARD)
- At a glance
- In detail
- Full Name:
- Probable ATP-dependent RNA helicase DDX58
- Alternative Names:
- DDX58, FLJ13599, DKFZp434J1111, DKFZp686N19181
- Function:
- Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV (By similarity).
- Death Domain Subfamily & Boundary Region:
-
CARD
:
1-87, 92-172
Species |
External DB
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Uniprot | DIP | STRING | KEGG | IntAct | MINT | ||
Human |
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O95786![]() |
DIP-35444N![]() |
O95786![]() |
hsa:23586![]() |
O95786![]() |
MINT-2799116![]() |
Mouse |
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Q6Q899![]() |
Q6Q899![]() |
mmu:230073![]() |
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Dog |
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E2RMV6![]() |
Structure | Species | Residue | Method | Expression | Purification | Column | Final condition | Final concentration | Reference | |
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4A2W |
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Duck | 1-933 | X-ray | BTI-TN-5B1-4 | Gel filtration | Superdex 200 | 20mM HEPES(pH 7.5), 150mM NaCl, 2.5mM MgCl2, 5mM betamercaptoethanol | 7mg/ml | 22000019 |
No information
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In vitro interaction | |||||||||||||
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DD1 | DD2 | Reference | |||||||||||
Family | DD1 | DD2 | Method | Species | Region | Expression | Species | Region | Expression | ||||
CARD | RIG-1 |
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NOD2 | His Fusion protein pull-down | Not specified | 1-205 | E.coli | Not specified | 1-301 | E.coli | 21690088 |
(
: click this icon to show interactions only between the two corresponding DDs)

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In vivo interaction | |||||||||||||
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Endogenous expression |
Overexpression | DD1 | DD2 | Reference | |||||||||
Family | DD1 | DD2 | Method | Species | Region | Species | Region | ||||||
CARD | RIG-1 |
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Caspase12 | Co-Immunofluorescence staining | Mouse embryonic fibroblast | Human | Not specified | Not specified | Not specified | 20818395 | |||
CARD | RIG-1 |
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Caspase12 | GST fusion protein pull-down | Mouse embryonic fibroblast | Human | Not specified | Not specified | Not specified | 20818395 | |||
CARD | RIG-1 |
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MAVS | Co-immunoprecipitation | HEK293 | Human | 1-604 | Human | Not specified | 16127453 | |||
CARD | RIG-1 |
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MAVS | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | Full length | 16177806 | |||
CARD | RIG-1 |
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MAVS | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | Not specified | 17020950 | |||
CARD | RIG-1 |
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MAVS | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not used | 21419663 | ||||
CARD | RIG-1 |
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MAVS | Fluorescence resonance energy transfer | Huh 7 | Not specified | Not specified | Not specified | Not specified | 19036819 | |||
CARD | RIG-1 |
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MDA5 | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Not specified | Not specified | 22328336 | |||
CARD | RIG-1 |
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NOD2 | Co-Immunofluorescence staining | HT29 | 21690088 | |||||||
CARD | RIG-1 |
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NOD2 | Co-Immunofluorescence staining | HEK293 | Not specified | Not specified | Not specified | Not specified | 21690088 | |||
CARD | RIG-1 |
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NOD2 | Co-immunoprecipitation | HEK293 | Not specified | Full length | Not specified | Full length | 21690088 | |||
CARD | RIG-1 |
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NOD2 | Co-immunoprecipitation | HEK293 | Not specified | Full length | Not specified | 1-301 | 21690088 | |||
CARD | RIG-1 |
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NOD2 | Co-immunoprecipitation | HEK293 | Not specified | 1-225 | Not specified | 1-301 | 21690088 | |||
CARD | RIG-1 |
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NOD2 | Co-immunoprecipitation | HEK293 | Not specified | 1-225 | Not specified | Full length | 21690088 |
(
: click this icon to show interactions only between the two corresponding DDs)

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Functional Role | |||||||||||
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Family | DD1 | DD2 | Method | Death-related | Death-unrelated | Reference | |||||
CARD | RIG-1 |
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MAVS | ELISA | Type 1 interferon induced | 16127453 | |||||
CARD | RIG-1 |
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MAVS | Luciferase assay | IFN-b induced | 16177806 | |||||
CARD | RIG-1 |
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NOD2 | Reporter gene assay | NF-kB activation block, IFN-b activation block | 21690088 |
(
: click this icon to show interactions only between the two corresponding DDs)
